Executable

    MBStar

(NOTE: THIS SOFTWARE IS FREELY AVAILABLE FOR ACADEMIC USE ONLY)
Download Instructions:

    Download the MBStar package.
    Unzip and extract all its files.
    Go to MBStar directory. Type 'chmod +x MBStar' and 'chmod +x MIL-Forest'.
    Type: ./MBStar -test < filename > -3utr < filename > -mir < filename > to execute MBStar.

Usage:

    Type: ./MBStar --help to see its usage.
    Parameters:
    test file: A Tab delimited two columns input file. First column contains the miRNA name (ex: >hsa-miR-155-5p) and the second column contains the Refseq id (ex: NM_182715) of mRNA. The last line of file should end with a '>'.
    3utr file: A fasta format file containing the 3'UTR sequences.
    mir file: A fasta format file containing the miRNA sequences.
    
Download zip file contains:
    hg19_3utr.txt: Human 3'UTR database file.
    mirbase_hsa_latest.txt: MicroRNA sequence file.
    positive_train_instance_index: An index file to train the classifier
    negative_train_instance_index: An index file to train the classifier
    positive_train40: Data file for training the classifier   
    negative_train40: Data file for training the classifier
    config.conf: Configuration file for classifier
    MBStar_example.txt: Some example interactions for MBStar

    The result is obtained by the following command:
    ./MBStar -test MBStar_example.txt -3utr hg19_3utr.txt -mir mirbase_hsa_latest.txt
    The result is saved in the file named 'MBStar_predicted_binding_sites.txt' 

Result:

		The result colomns are as follows:
			col1: miRNA Name
			col2: mRNA Name
			col3: Binding sites with flanking regions
			col4: miRNA Sequence
			col5: Seed type
			col6: Binding position

	
Please note:

	The program is compiled under linux system.