Executable

MultiMiTar

Download Instructions:

• Download the MultiMiTar package.
• Unzip and extract all its files.
• Go to libsvm-2.88 directory. Use the command 'make'.
• Go to MultiMiTar directory. Type 'chmod +x MultiMiTar'.
• Type: ./MultiMiTar -test < filename > -3utr < filename > -mir < filename > to execute MultiMiTar.

Usage:

• Type: ./MultiMiTar --help to see its usage.
  

Parameters:

• test file: A Tab delimited two columns input file. First column contains the miRNA name (ex: >hsa-miR-1) and the second column contains the Refseq id (ex: NM_018362) of mRNA.
  
• 3utr file: A fasta format file containing the 3'UTR sequences.
  
• mir file: A fasta format file containing the miRNA sequences.



Download zip file contains:

• hg18_3utr.txt: Human 3'UTR database file.
  
• hsa_mirbase_list.txt: MicroRNA sequence file.
  
• biovalidated_positive_examples.txt: Input example file containing biologically validated positive examples.
  

The result is obtained by the following command:

• ./MultiMiTar -test biovalidated_positive_examples.txt -3utr hg18_3utr.txt -mir hsa_mirbase_list.txt

  Output files:

• miR_rank_list.html: The file contains rank list based on miRNAs
  
• mRNA_rank_list.html: The file contains rank list based on mRNAs
  
• default_rank_list.html: The file contains unsorted predictions