Welcome to MBSTAR Executable

Executable

MBStar (Click here to Download)

Download Instructions:

• Download the MBStar package.
• Unzip and extract all its files.
• Go to MBStar directory. Type 'chmod +x MBStar' and 'chmod +x MIL-Forest'.
• Type: ./MBStar -test < filename > -3utr < filename > -mir < filename > to execute MBStar.

Usage:

• Type: ./MBStar --help to see its usage.
  

Parameters:

test file: A Tab delimited two columns input file. First column contains the miRNA name (ex: >hsa-miR-155-5p) and the second column contains the Refseq id (ex: NM_182715) of mRNA. The last line of file should end with a '>'.
3utr file: A fasta format file containing the 3'UTR sequences.
mir file: A fasta format file containing the miRNA sequences.


Download zip file contains:

• hg19_3utr.txt: Human 3'UTR database file.
  
• mirbase_hsa_latest.txt: MicroRNA sequence file.
  
• positive_train_instance_index: An index file to train the classifier.
  
• negative_train_instance_index: An index file to train the classifier.
  
• positive_train40: Data file for training the classifier.
  
• negative_train40: Data file for training the classifier.
  
• config.conf: Configuration file for classifier.
  
• MBStar_example.txt: Some example interactions for MBStar.
  
  
The result is obtained by the following command:
./MBStar -test MBStar_example.txt -3utr hg19_3utr.txt -mir mirbase_hsa_latest.txt
The result is saved in the file named: MBStar_predicted_binding_sites.txt

Result:

The result colomns are as follows:


• col1: miRNA Name
  
• col2: mRNA Name
  
• col3: Binding sites with flanking regions
  
• col4: miRNA Sequence
  
• col5: Site type
  
• col6: Binding position